32nd Meeting of the Danish Society for Flow Cytometry

1. Cell Sorting - Scientific Symposium and Workshops (21-22 April 2004).
   
2. Generalforsamling - Annual General Meeting (21 April 2004).

Symposium: Cell sorting

Auditorium 2, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen Ø.
Welcome (11:00-11:10). Jørgen K. Larsen and Jens Peter Stenvang, organizers, DSFCM.

All abstracts.

Session I: Flow sorting (11:10-14:40).

• Technologies and applications.
  • 11:10-11:40. Phenotyping and gene expression profiling of human myeloid progenitors using FACSAria for cell sorting. Tor Olofsson, Dept. of Hematology, Institute for Laboratory Medicine, Lund University C14, BMC, S-221 84 Lund, Sweden.
  • 11:40-12:10. Methodological aspects of gene expression in high-speed flow sorted stem cells (side population cells) from mouse bone marrow (abstract). Oleg Tsinkalovsky, Stem Cell Research Group, Gade Institute, Department of Pathology, Haukeland University Hospital, Bergen, Norway.
  • 12:10-12:40. Flow cytometric sexing of mammalian spermatozoa (abstract). Preben Christensen, Section for Reproduction, Royal Veterinary and Agricultural University, Frederiksberg.
  • 12:40-12:55. Cell surface expression of prion protein (PrPc) on subsets of peripheral blood cells from sheep of different genotypes (abstract). Jacob Ohm Eriksen, Danish Institute for Food and Veterinary Research, Department of Veterinary Diagnostics and Research, Copenhagen.
  • 13:55-13:25. Cell Sorting with Pathogens - Safety Precautions in Flow Sorting at Containment Level 3 (abstract). Julie Rogers, Health Protection Agency (Porton Down), Salisbury, Wiltshire SP4 OJG, UK
• Sandwich break (13:25-13:40).
• Hardware & software.
  • 13:40-14:10. BD FACS Aria and FACS Diva cell sorters. Mark Dessing, BD Biosciences A/S, Brøndby.
  • 14:10-14:40. DakoCytomation MoFlo cell sorter. George Malachowski, DakoCytomation, Fort Collins, Colorado, USA.

Session II, parallel A: Commercial exhibition - with refreshments/coffee/tea (14:40-15:20).

• AH Diagnostics A/S, Aarhus (StemCell Technologies). Helle Sørensen.
• BD Biosciences A/S, Brøndby. Lene Askjær Sørensen and Sverker Segren.
• Bie & Berntsen A/S, Rødovre (Kingfisher). Louise Norman.
• Biotech Line A/S, Slangerup (Miltenyi Biotech). Peter Simon, Christina El-Naaman and Pernille Nørskov.
• Brock & Michelsen A/S, Birkerød (Zeiss /PALM Microlaser Technologies). Per Geltzer.
• DakoCytomation Norden A/S. Roger Festin.
• DFA Instruments A/S, Copenhagen (Nikon /Molecular Machines & Industries /SLμCUT Laser Microdissection System). Poul Dam Hansen.
• R&D Systems Europe Ltd, Abingdon Science Park, United Kingdom. Karen Pugh (Pilkington).

Session II, parallel B: Generalforsamling (Annual General Meeting, in Danish) (14:50-15:20)

• Dagsorden: Valg af dirigent, formandsberetning, indkomne forslag*, valg til bestyrelsen**, revideret regnskab, budget, kontingent*, forretningsorden, hjemmeside og e-mail, kvalitetskontrol, legat*, planer for kommende møder, eventuelt.
  *Bestyrelsen foreslår, at det årlige kontingent ændres til 0 kr. for ordinære medlemmer fra og med år 2005, men fortsættes med 1.000 kr. for firmamedlemmer. I samme forbindelse foreslås legatet afskaffet.
  **På valg er posterne for 3 bestyrelsesmedlemmer (Jørgen K. Larsen, Hans Jürgen Hoffmann og Carl-Henrik Brogren), 1 suppleant (Tine Hoppe) og 1 revisor (Jacob Larsen). Alle er villige til genvalg.
  

Session III: Immunomagnetic sorting. (15:20-16:20)

• Technologies and applications.
  [Cancelled: Immunomagnetic cell sorting - Comparison of methods (abstract). Jan Pravsgaard Christensen, Institute for Medical Microbiology and Immunology, Panum Institute, University of Copenhagen, Copenhagen.]
• Hardware & software.
  • 15:20-15:35. Miltenyi Biotech MACS beads. Peter Simon, Biotech Line A/S, Slangerup.
  • 15:35-15:50. Cell separation or pre-enrichment prior to FACS by StemCell Technologies. Michael Simonds, StemCell Technologies /AH Diagnostics A/S, Aarhus.
  • 15:50-16:05. Immunomagnetic sorting with R&D Systems MagCellect. Frances Clay, R&D Systems Europe Ltd, Abingdon Science Park, United Kingdom.
  • 16:05-16:20. BD IMag Cell Separation System. Sverker Segren, BD Biosciences A/S, Brøndby.

Session IV: Laser microdissection. (16:20-18:10)

• Technologies and applications.
  • 16:20-16:40. Laser microdissection in proteomics (abstract). Pavel Gromov, Institute of Cancer Biology, Danish Cancer Society, Copenhagen.
  • 16:40-17:00. Micro laser dissection on live cells (abstract). Renate Burgemeister, PALM Service & Applications Laboratory, Bernried, Germany.
  • 17:00-17:20. Detection of single cancer cells in patient blood and bone marrow (abstract). Siri Tveito, Dept. of Tumor Biology, Norwegian Radium Hospital, Oslo, Norway.
  • 17:20-17:40. Cytogenetic investigation of laser-capture microdissected carcinoma in situ cells from testicular tissue (abstract). Anne Marie Ottesen, Dept. of Growth and Reproduction, Rigshospitalet, Copenhagen.
• Hardware and software.
  • 17:40-17:55. Zeiss PALM laser microdissection system. Per Geltzer, Brock & Michelsen A/S, Birkerød.
  • 17:55-18:10. NIKON /MMI SLμCut laser microdissection system. Poul Dam Hansen, DFA Instruments A/S, Copenhagen.

Session V: Commercial exhibition (continued) - with tapas/buffet and refreshments/wine (18:10-)

Workshops: Cell Sorting

At different locations in the region, practical demonstration of flow sorters, immunomagnetic sorting systems, and laser microdissection instruments. Workshop sessions of about 1½ hours duration (09:00-10:30, 10:30-12:00, 12:30-14:00) with a maximum number of 5-7 participants.
• Flow sorting
  • A) BD FACS Aria (09:00-10:30, 12:30-14:00). Mark Dessing, BD Biosciences A/S /Lars Ryder, Tissue Typing Laboratory, Dept. of Clinical Immunology, Rigshospitalet, Tagensvej 20, 2200 Copenhagen N (Building 75, Entrance 7501, go downstairs, then to the right). Site Map.
  • B) BD FACS SE Diva (09:00-10:30, 12:30-14:00). Henrik Holst, BD Biosciences A/S /Jørgen K. Larsen, Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, 2100 Copenhagen Ø (Building 7, 1. sal). Site Map. Road Map.
  • C) DakoCytomation MoFlo (09:00-10:30, 10:30-12:00, 12:30-14:00). George Malachowski and Steven Le Moënic, DakoCytomation A/S, Produktionsvej 40, 2600 Glostrup (Ask at the reception). Site Map. Road Map.
• Immunomagnetic sorting.
  • D) Miltenyi Biotech MACS beads (09:00-10:30, 10:30-12:00). Peter Simon, Christina El-Naaman and Pernille Nørskov, Biotech Line A/S /Jørgen K. Larsen, Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, 2100 Copenhagen Ø (Building 7, 1. sal). Site Map. Road Map.
  • E) R&D Systems MagCellect (10:30-12:00). Frances Clay, R&D Systems Europe Ltd. /Jørgen K. Larsen, Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, 2100 Copenhagen Ø (Building 7, 2. sal). Site Map. Road Map.
• Laser microdissection.
  • F) Arcturus PixCell II (10:30-12:00). Anette Poulsen, Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, 2100 Copenhagen Ø (Building 7, 1. sal). Site Map. Road Map.
  • G) Nikon /MMI SLμCUT (12:30-14:00). Poul Dam Hansen, DFA Instruments A/S, Copenhagen /Jørgen K. Larsen, Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, 2100 Copenhagen Ø (Building 7, 2. sal). Site Map. Road Map.
  • H) Zeiss PALM (09:00-10:30). Per Geltzer, Brock & Michelsen A/S /Pavel Gromov, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, 2100 Copenhagen Ø (Building 4, 3. sal). Site Map. Road Map.

• Please, register for the meeting by e-mail to Jørgen K. Larsen and indicate your preferences for participation in workshops.
• All are welcome, members of the Danish Medical Society and everyone else who might be interested! There is no registration fee.
• The workshops are limited to a certain maximum number of participants. The organizers reserve the right to decide the assignment of participants to the particular workshops. Be aware that the workshops program may be changed with a short notice.
• With respect to lodging you have to make your own arrangements.
• If you have suggestions for the program, please contact one of the organizers: Jørgen K. Larsen and Jens Peter Stenvang.

• AH Diagnostics (StemCell Technologies).
• BD Biosciences A/S.
• Bie & Berntsen A/S.
• Biotech Line A/S (Miltenyi Biotech).
• Brock & Michelsen A/S (Zeiss /PALM Microlaser Technologies).
• DakoCytomation Norden A/S (sponsoring Oleg Tsinkalovsky).
• Danish Medical Society (sponsoring Julie Rogers)
• DFA Instruments A/S (NIKON /Molecular Machines & Industries).
• R&D Systems Europe Ltd.