Healthy volunteers with known response to
exposure in swine confinement buildings were exposed for three hours with work
and rest. Blood and bronchoalevolar lavage samples for flow cytometry were
taken at baseline one week before exposure and 24 hours after exposure. The
response of blood monocytes and dendritic cells was studied by 4 colour stain
combinations.
Dendritic cells were identified with the
BD antibody set, identified as Lin1- HLADR+ cells that were resolved into DC1
and DC2 in a CD11c CD123 dotplot. CD11c, CD123 and HLADR were evaluated by
probability binning. All parameters were reduced after exposure in SCB,
significantly only for CD123 (p=0,013). In multivariate distribution analyses
the difference was increased by inclusion of CD11c and HLA DR (p=0,008), but
not by analysis of only CD123 and CD11c.
When identifying monocytes as CD14+HLA DR+
cells to investigate a change in CD80 and CD86 expression, the extent of HLA DR
and CD14 expression rather than the expression of CD86 and CD80 varied. Changes
of monocyte expression of CD14 and HAL DR may be good markers of stress, and
were investigated by multivariate distribution analyses. In the exposure model,
CD14 did not vary, whereas