Danish Society for Flow Cytometry

20th Meeting - Monday, December 14, 1998

Minisymposium on Flow Cytometry in Microbiology

Technical aspects and some applications in agriculture, environmental and industrial sciences

Organizer: Carl-Henrik Brogren

Location:

Institute of Food Safety and Toxicology, Building C, Room 102 (C102)

Danish Veterinary and Food Administration, Mørkhøj Bygade 19, DK-2860 Søborg

Telephone: (45) 3395 6000. Internet: http://www.vfd.dk

10:00-10:30 Registration*: Contact first the Reception Desk, where you will be directed to the meeting room C102 for registration. Coffee and tea will be served.

10:30-10:35 Wellcome: Introduction to the Minisymposium (Carl-Henrik Brogren, DSFCM)

Session 1: History and future (chairman: Jørgen K. Larsen, DSFCM president)

10:35-11:00 Knud Rasmussen, Department of Microbiology, Technical University of Denmark, Lyngby: Bacteria and Flow Cytometry - A Historical Review.

11:00-11:15 Ulrik Darling Larsen, Bio/Chemical Microsystem Group, Mikroelektronik Centret (MIC), Technical University of Denmark, Lyngby: Microchip Coulter Particle Counter.

11:15-11:30 Carl-Henrik Brogren, Department of Microbiological Safety, Institute of Food safety and Toxicology, Danish Veterinary and Food Administration, Søborg: MACS-FCM - immunomagnetic separation and immunospecific detection in bacterial FCM.

11:30-12:00 Lunch buffet in Room "Laden 1"

Session 2: Basic microbiology (chairman: Knud Rasmussen, Department of Microbiology, Technical University of Denmark, Lyngby)

12:00-12:40 Øystein Rønning, Optoflow, Oslo, Norway (sponsored by In Vitro/OptoFlow): MICROCYTE, a compact, easy-to-use flow cytometer for detection, characterisation and enumeration of microorganisms.

12:40-12:55 Birgitte Bjørn Budde, Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg: Flow cytometry used to determine the antimicrobial effect of bacteriocins.

12:55-13:10 Flemming G. Hansen, Department of Microbiology, Technical University of Denmark, Lyngby: Using flow cytometry in the study of the bacterial cell cycle.

13:10-13:25 Ulrik von Freiesleben, Department of Microbiology, Technical University of Denmark, Lyngby: Secondary replication strat sites in IHF mutants.

13.25-13.40 Posters - Coffee/tea

Session 3: Environmental and industrial microbiology (chairman: Mogens Jakobsen, Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg )

13:40-14:20 Philippe Lebaron, Laboratoire ARAGO, Banyuls-sur-Mer, France (sponsored by Becton-Dickinson): New insight in the detection of bacteria by cytometry techniques in regards to their physiological state and identification?

14:20-14:35 Niels Henrik Borch, Fresh Water Biology Laboratory, University of Copenhagen, Hillerød: FCM and aquatic microbiology: pitfalls and prospects.

14:35-14:50 Morten Søndergaard, Fresh Water Biology Laboratory, University of Copenhagen, Hillerød: Use of FCM in phytoplankton studies.

14:50-15:05 Marie Roennenkamp Hansen, Bacillus Development, NOVO-Nordisk A/S, Bagsværd: Flow cytometry applied to Bacillus fermentation.

15.05-15.20 Posters - Aperitif/soft drinks

Session 4: Food microbiology (chairman: Jørgen Schlundt, Department of Microbiological Safety, Institute of Food safety and Toxicology, Danish Veterinary and Food Administration, Søborg)

15:20-16:00 Gerhard Nebe-von-Caron, Unilever Research, Bedfordshire, England (sponsored by RAMCON/ Beckman Coulter Corp.): Viable But Non Culturable Bacteria in Flow Cytometry.

16:00-16:15 Lars Uhre Guldfeldt, Alfred Jørgensens Laboratorium, Frederiksberg: Use of flow cytometry for estimation of the viability and vitality of yeast starter cultures.

16:15-16:30 Birthe Hald, Department of Poultry, Fish, and Fur Animals, Danish Veterinary Laboratory, Århus: Flow cytometric evaluation of fluorescent formazan (CTC) deposits as a viability measure in started Campylobacter jenuni compared with culture techniques.

16:30-16:45 Peter Saadbye, Department of Microbiological Safety, Institute of Food Safety and Toxicology, Danish Veterinary and Food Administration, Søborg: Physiological State of heat-stressed Salmonella typhimurium determined by flow cytometry.

16:45-17:00 General discussion (chairman: Carl-Henrik Brogren, DSFCM)

Presented posters can be discussed.

Final remarks (webmaster Jens Peter Stenvang, DSFCM vice-president)

Forming an internet-usergroup - microbiol@flowcytometri.dk ?

*Registration: Please, register before December 1, 1998. The number of participants will be limited to 75. Members of DSFCM have priority. No registration fee.

Registration to by e-mail: chb@vfd.dk, or by fax: (45) 3395 6696 /att. Carl-Henrik Brogren.

Posters: A limited number of posters may be presented. Indicate in your registration, if you wish to present a poster.

Exhibition: A small area will be dedicated to exhibitions from companies for topics related to the program.

Sponsors: DSFCM greatly acknowledges the following sponsors supporting this minisymposium:

Becton-Dickinson Denmark, Ramcon/Beckman-Coulter Corp., In Vitro/Optoflow,

AH-Diagnostics/Miltenyi, KEBO Lab A/S, and Bie & Berntsen/Molecular Probes.

President Jørgen K. Larsen, Finsen Laboratory, Finsen Center, Rigshospitalet, Dept. 8621, Strandboulevarden 49, DK-2100 Copenhagen Ø, Denmark. Phone (45) 3545 5751. Fax (45) 3538 5450. E-mail j.k.larsen@finsenlab.dk.

Vice-president Jens Peter Stenvang, Bartholin Institute, Kommunehospitalet, Øster Farimagsgade 5, DK-1353 Copenhagen K, Denmark. Phone (45) 3338 3857. Fax (45) 3393 8566. E-mail stenvang@biobase.dk.

Meetings coordinator Carl-Henrik Brogren, Division of Microbiological Safety, Institute of Food Safety and Toxicology, Danish Veterinary and Food Administration, Mørkhøj Bygade 19, DK-2860 Søborg. Phone (45) 3395 6184. Fax (45) 3395 6696. E-mail chb@vfd.dk.

Secretary Søren Pedersen, Dept. of Clinical Genetics, Bartholin Building, Aarhus University Hospital, DK-8000 Aarhus C. Phone (45) 8942 1674. Fax (45) 8949 4370. E-mail spe@biobase.dk.

Treasurer Bjarne Møller, Dept. of Clinical Immunology, Odense University Hospital, DK-5000 Odense C, Denmark. Phone (45) 6541 3576. Fax (45) 6612 7975. E-mail bjarne.moeller@ouh.dk.

Abstracts are available, but not in electronic format.